A growing role for reverse transcription

A Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype

Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the ra...

Development of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses

Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Primer tRNAs for reverse transcription.

Both retroviruses and long terminal repeat (LTR) retrotransposons use cellular tRNAs as primers for reverse transcription during their replication cycles. In retroviruses, primer tRNA is selectively packaged into the virion, where it is placed onto the primer binding site (PBS) of the viral RNA genome and used to prime the reverse transcriptase (RT)-catalyzed synthesis of minus-strand cDNA. Stu...

Minireview Primer Trnas for Reverse Transcription

Primer tRNAs for reverse transcription.